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During subsequent resuscitation
with the core criterion for stopping lysis being a clear and transparent solution
The enzymes (such as collagenase and neutral protease) in the dissociation solution lose ≤10% of their activity when stored at 4℃ for a short period (≤3 hours)
with each tube containing 180-220mg of tissue and corresponding to 240μL of dissociation solution + 2760μL of RPMI 1640 medium
endotoxin-free water to maintain assay performance
Endotoxin Assay Water | LAL Reagent Water | Water for Bacterial Endotoxins Test Size:500mL/bottle During subsequent resuscitationEndotoxin Assay Water, also known as Water for Bacterial Endotoxins Test, is a high purity, sterile, and pyrogen free water specifically manufactured for use in Limulus Amebocyte Lysate (LAL) assays and other bacterial endotoxin testing applications. It is rigorously validated to meet the specifications of USP <85>, EP 2. 6. 14, and other relevant pharmacopoeial standards, ensuring it is free of detectable endotoxins and any interfering substances.
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