Ascorbate Peroxidase Microplate Assay Kit Proteins and Enzymes as well as a polyglutamine

as well as a polyglutamine region at residues 35 to 46

Within each well added 50uL Stop solution

Specific anti human TNF - &alpha  Capture antibody was pre coated on a high affinity microplate

and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm)

(2007) Immunity 26:371

Ascorbate Peroxidase Microplate Assay Kit Proteins and Enzymes as well as a polyglutamineProduct Specification Detection Type Colorimetric Method General Notes The Dye in the kit is easy to oxidise and deteriorate after dissolving, so it needs to be mixed and used on the spot, and is not recommended for storage. Instructions Fluid of tissue extract, cell lysis Storage Temp. Transportation and storage at 4 , 6months Applications Used for the detection and quantification of Ascorbate Peroxidase activity.