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mount brain sections upon adhesive microscope slides
· What is the shelf life of the 4% PFA
Jing-Ruei Lin
Fix of sections/organotypic slice cultures: Add to sections/slices (the volume of 4% PFA should be approximately 10 times the volume of the tissue) and incubate for 0
Figure: Bioenno DAB Substrate Kit employed together with the Bioenno BDHC Substrate Kit
Slide Mount for Fluorescent Labeling (Cat#032019) Slide Mount mount brain sections upon adhesiveAqueous Antifade Mountant for Fluorescent LabelingLaboratory Use Only, Store at 20C to 25CThe water based Slide MountTM is specially formulated for preserving fluorescent labeling in brain sections. This mounting medium contains antifade reagents that provide protection against fading or photobleaching of commonly used fluorophores. It has been proven to preserve fluorescence signaling on paraformaldehyde (PFA) fixed brain sections and can be used in
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